A DNA sequence modified with methylene blue (MB) was covalently bound to the electrochemical strip and used to detect the selected target miRNA-21. In the present study, miRNA-21 was selected as a model sequence, due to its role in prostate, breast, colon, pancreatic, and liver cancers. In this work, an electrochemical detection platform for miRNA based on a screen-printed gold electrode was developed. The determination of these targets may be affected by the different physicochemical parameters of the specimen of interest. They often play an important role in the early diagnosis and prognosis of cancer, and the development of simple and effective analytical methods would be of pivotal importance for the entire community. They have been found in liquid biopsy samples, including various bodily fluids.
MicroRNAs (miRNAs) are clinical biomarkers for various human diseases, including cancer.
